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Identity No. |
Subject (kg) |
Weight (cm) |
Height (%) |
W/H index kcal/day |
Energy Intake kcal/kg/day |
(delta) Weight (kg) |
44 | 54.1 | 168.5 | 82.7 | 3,200 | 59.1 | 0.2 |
45 | 55.8 | 164.6 | 86.0 | 3,200 | 57.3 | - 0.3 |
46 | 62.7 | 164.0 | 98.7 | 3,200 | 51.0 | 0.1 |
48 | 57.8 | 166.7 | 89.5 | 3,200 | 55.4 | 0.6 |
49 | 64.0 | 161.7 | 103.7 | 3,200 | 50.0 | 0.1 |
51 | 61.0 | 161.0 | 99.8 | 3,200 | 52.5 | - 0.8 |
52 | 68.4 | 173.8 | 98.6 | 3,200 | 46.8 | - 0.2 |
53 | 64.5 | 174.5 | 92.5 | 3,200 | 49.6 | - 0.2 |
54 | 67.0 | 169.5 | 101.2 | 3,200 | 47.8 | - 0.2 |
55 | 64.8 | 170.5 | 96.9 | 3,200 | 49.4 | - 0.5 |
56 | 56.7 | 164.0 | 90.1 | 3,200 | 56.4 | - 0.1 |
57 | 51.8 | 161.8 | 83.9 | 3,200 | 61.8 | 0.8 |
58 | 55.8 | 160.0 | 92.2 | 3,200 | 57.3 | 0 |
59 | 73.2 | 180.8 | 97.6 | 3,200 | 47.8 | - 1.5 |
60 | 63.7 | 168.6 | 96.7 | 3,200 | 50.2 | 0.5 |
Mean | 62.4 | 166.1 | 96.7 | 3,231 | 52.4 | - 0.13 |
SD | 6.7 | 6.5 | 7.1 | 89 | 5.0 | 0.73 |
Anthropometric measurements such as body weight, height, waist, gluteal and mid-upper arm circumference, triceps fat-fold and subscapular fat-fold, thorax circumference and cranial circumference were obtained on days 1 and 11.
Summary of Main Results
Energy intake
The subjects received a mean daily energy intake of 3,247 ± 104 kcal/day, or 52 ± 5 kcal/kg/day.
Weight change Negligible, non-significant mean body weight changes were observed. About half of the individuals gained weight and the rest lost it, with an overall mean equal to - 0.128 ± 0.729.
Urinary nitrogen intake: The mean urinary nitrogen excretion computed for the last five days was 143.5 ± 14 mg/kg/day.
TABLE 2 Mean Daily Nitrogen Balances Resulting from a Chilean Mixed Diet Consumed for 10 Daysa
Subject Identity No |
N Intake |
Urinary N |
Faecal N |
N Balance |
mg N/kg/day |
||||
1 | 183 | 139.3 | 35.7 | + 3.0 |
2 | 183 | 165.9 | 19.4 | - 73 |
3 | 183 | 156.7 | 31.5 | - 10.2 |
4 | 183 | 136.2 | 36.9 | + 4 9 |
5 | 182 | 144.0 | 25.0 | + 8.0 |
6 | 183 | 136.6 | 33.2 | + 8.2 |
7 | 183 | 156.2 | 28.1 | - 6.3 |
8 | 183 | 135.0 | 37.6 | + 54 |
9 | 182 | 123.6 | 44.1 | + 9 3 |
10 | 182 | 118.7 | 22.7 | + 35.6 |
11 | 183 | 126.4 | 34.2 | + 174 |
12 | 183 | 141.2 | 44.3 | - 75 |
13 | 183 | 158.8 | 16.7 | + 2.5 |
14 | 182 | 141.0 | 29.4 | + 6.6 |
15 | 183 | 142.6 | 28.0 | + 74 |
16 | 183 | 152.0 | 36.7 | - 10.7 |
17 | 183 | 156.1 | 37.6 | - 15.6 |
18 | 182 | 159.3 | 30.5 | - 12 8 |
19 | 185 | 159.6 | 34.5 | - 14.1 |
20 | 183 | 164.9 | 27.9 | - 1.6 |
21 | 183 | 137.4 | 35.4 | + 5.2 |
22 | 182 | 142.5 | 35.1 | - 0.6 |
24 | 187 | 149.6 | 23.7 | + 8.7 |
25 | 183 | 125.4 | 35.0 | + 17.6 |
26 | 182 | 135.6 | 30.4 | + 110 |
27 | 182 | 158.7 | 31.9 | - 13.6 |
28 | 182 | 129.0 | 34.8 | + 13.2 |
29 | 182 | 124.2 | 27.3 | + 25.5 |
30 | 182 | 147.8 | 28.4 | + 0.8 |
31 | 182 | 146.0 | 30.3 | + 07 |
33 | 183 | 133.8 | 21.6 | + 22.6 |
35 | 182 | 159.5 | 20.3 | - 2.8 |
36 | 183 | 133.6 | 23.5 | + 20.9 |
37 | 183 | 155.1 | 24.3 | - 1.4 |
38 | 182 | 153.7 | 23.1 | + 0.2 |
39 | 182 | 158.4 | 29 2 | + 10.6 |
42 | 183 | 141.4 | 20.6 | + 16.0 |
TABLE 2. (continued)
Subject Identity No |
N Intake |
Urinary N |
Faecal N |
N Balance |
mg N/kg/day |
||||
43 | 183 | 149.6 | 30.3 | - 1.9 |
44 | 183 | 153.3 | 22.6 | + 2.1 |
45 | 183 | 142.7 | 20.3 | + 15.0 |
46 | 183 | 155.8 | 19.2 | + 3.0 |
48 | 183 | 154.7 | 26.7 | + 3.4 |
49 | 183 | 114.2 | 36.0 | + 27.8 |
51 | 183 | 129.2 | 25.5 | + 23.3 |
52 | 183 | 147.8 | 24.5 | + 5.7 |
53 | 183 | 127.0 | 46.5 | + 4.5 |
54 | 183 | 168.4 | 50.3 | - 10.7 |
55 | 183 | 144.4 | 25.1 | + 8.5 |
56 | 182 | 119.4 | 41.8 | + 15.8 |
57 | 183 | 113.4 | 37.2 | + 27.4 |
58 | 183 | 132.5 | 30.1 | + 15.4 |
59 | 183 | 160.0 | 16.2 | + 1.8 |
60 | 183 | 146.1 | 21.9 | + 10.0 |
Mean | 183 | 143.5 | 29.3 | + 5.8 |
SD | 14.1 | 7.3 | 11.7 |
Faecal nitrogen The faecal N excretion was 29.3 ± 7.3 mg/kg/day. It is important to point out that this value is of the same magnitude found in our previous experiments in which male subjects consumed the same mixed diet in short-term and long-term experiments.
Nitrogen balance: The mean nitrogen balance for the population studied was + 5.8 ± 11.7 mg N/kg/day (table 4).
Biochemical values in blood: All the parameters studied at the onset of the study fell within the normal range of values for young adult male subjects.
Anthropometric measurements: The anthropometric measurements made on days 1 and 11 failed to reveal significant changes in the parameters studied.
TABLE 3. Biochemical Measurements
Subject Identity No |
Total Protein (g/dl) |
Albumin (g/dl) |
Globulin (g/dl) |
A/G Index |
Haematocrit (%) |
Haemoglobin (g/dl) |
1 | 7.7 | 4.8 | 2.9 | 1.6 | 49.3 | 16.9 |
2 | 6.3 | 4.0 | 2.3 | 1.7 | 43.0 | 14.3 |
3 | 7.3 | 5.0 | 5.5 | 1.9 | 44.5 | 15.3 |
4 | 6.3 | 4.2 | 2.1 | 2.0 | 45.2 | 15.3 |
5 | 7.0 | 5.2 | 1.8 | 2.8 | 46.9 | 16.1 |
6 | 6.3 | 4.0 | 2.2 | 1.8 | 42.8 | 14.5 |
7 | 6.9 | 5.1 | 1.8 | 2.8 | 46.7 | 16.1 |
8 | 6.1 | 4.4 | 1.7 | 2.5 | 46.8 | 16.5 |
9 | 6.7 | 4.4 | 2.3 | 1.9 | 44.8 | 15.5 |
10 | 7.3 | 4.9 | 2.4 | 2.1 | 44.1 | 15.4 |
11 | 7.1 | 4.5 | 2.6 | 1.8 | 45.0 | 15.6 |
12 | 7.1 | 4.9 | 2.2 | 2.3 | 39.1 | 13.6 |
13 | 6.5 | 4.1 | 2.3 | 1.8 | 46.5 | 16.0 |
14 | 6.3 | 3.9 | 2.4 | 1.7 | 43.6 | 15.0 |
15 | 6.7 | 4.5 | 2.2 | 2.0 | 48.1 | 16.4 |
16 | 7.0 | 4.7 | 2.3 | 2.1 | 45.4 | 16.1 |
17 | 6.7 | 4.7 | 2.0 | 2.3 | 45.7 | 15.7 |
18 | 7.7 | 5.2 | 2.5 | 2.1 | 44.4 | 15.4 |
19 | 6.8 | 4.4 | 2.8 | 1.8 | 45.8 | 15.9 |
20 | 6.7 | 3.4 | 2.2 | 1.1 | 46.7 | 16.1 |
21 | 6.9 | 4.2 | 2.7 | 1.5 | 43.3 | 14.4 |
22 | 6.3 | 4.4 | 1.9 | 2.3 | 46.8 | 15.3 |
24 | 6.5 | 4.1 | 2.4 | 1.7 | 49.6 | 16.6 |
25 | 7.0 | 4.2 | 2.8 | 1.5 | 43.1 | 14.9 |
26 | 7.0 | 4.7 | 2.4 | 2.0 | 47.7 | 16.2 |
27 | 7.1 | 5.0 | 2.1 | 2.5 | 47.0 | 16.4 |
28 | 6.0 | 4.4 | 1.6 | 2.7 | 49.1 | 16.8 |
29 | 6.5 | 4.7 | 1.7 | 2.8 | 46.5 | 16.0 |
30 | 6.5 | 4.1 | 2.4 | 1.7 | 44.5 | 15.2 |
33 | 7.6 | 4.7 | 2.9 | 1.6 | 45.4 | 15.3 |
35 | 7.8 | 4.6 | 3.2 | 1.4 | 45.6 | 14.9 |
36 | 7.0 | 4.6 | 2.4 | 1.9 | 42.2 | 13.5 |
37 | 7.0 | 4.4 | 2.5 | 1.8 | 45.0 | 15.5 |
38 | 7.0 | 4.6 | 2.4 | 1.9 | 43.8 | 14.7 |
39 | 7.6 | 4.6 | 3.0 | 1.5 | 43.4 | 15.0 |
42 | 6.2 | 3.9 | 2.3 | 1.7 | 41.8 | 14.1 |
43 | 7.0 | 4.9 | 2.1 | 2.3 | 46.2 | 16.3 |
44 | 6.2 | 4.4 | 1.8 | 2.4 | 39.6 | 13.3 |
45 | 6.4 | 3.9 | 2.4 | 1.6 | 42.2 | 14.1 |
TABLE 3. (continued)
Subject identity No |
Total a Protein (g/dl) |
Albumin (g/dl) |
Globulin (g/dl) |
A/G Index |
Haematocrit (%) |
Haemoglobin (g/dl) |
46 | 6.7 | 4.6 | 2.0 | 2.3 | 38.1 | 1 3.0 |
48 | 7.0 | 4.1 | 2.9 | 1.4 | 47.8 | 1 6.5 |
49 | 6.8 | 3.4 | 3.4 | 1.0 | | |
51 | 7.0 | 4. 1 | 2.9 | 1 .4 | 47.2 | 15.8 |
52 | 7.3 | 4.1 | 3.2 | 1.3 | 43.6 | 14.9 |
53 | 6.8 | 3.7 | 3.1 | 1.2 | 44.4 | 14.7 |
54 | 7.2 | 3.3 | 3.9 | 0.8 | | |
55 | 6.2 | 4.4 | 1 .8 | 2.4 | 39.8 | 1 3.4 |
56 | 6.8 | 3.3 | 3.5 | 0.9 | 43.4 | 14.4 |
57 | 7.4 | 4.0 | 3.4 | 1 .2 | 43.8 | 1 5.7 |
58 | 6.8 | 3.5 | 3.2 | 1.1 | 46.2 | 16.5 |
59 | 7.4 | 3.9 | 3.5 | 1.1 | | |
60 | 7.6 | 4. 1 | 3.5 | 1 .2 | 41 .6 | 1 4.7 |
Mean | 6.8 | 4.3 | 2.6 | 2.0 | 44.7 | 1 5.3 |
SD | 0.5 | 0.5 | 0.7 | 1.5 | 2.6 | 1 0 |
TABLE 4. Distribution of Nitrogen Balance
N Balance (mg/kg) | No. | % | Cumulative | |
No. | % | |||
>= + 10.0 | 17 | 32.1 | 17 | 32.1 |
+ 5.0 to 9.9 | 10 | 18.9 | 27 | 50.9 |
0.0 to 4.9 | 11 | 20.7 | 38 | 71.7 |
- 5.0 to - 0.1 | 5 | 9.4 | 43 | 81.1 |
- 10.0 to - 5.1 | 3 | 5.7 | 46 | 86.8 |
< - 10.0 | 7 | 13.2 | 53 | 100.0 |
Conclusions and Comments
Mean daily N balance was slightly positive for the group, but seven of 53 individuals mantained on 1.1 9 of protein per kg were in significant negative N balance (lower than - 10 mg N/kg/day).
Subjects with the largest positive N balance corresponded to those with highest energy intakes.
The faecal N excretion level was comparable to that in our previous data using similar diets and subjects.
Subjects appeared to be in energy balance based on weight and anthropometric measurements.
A predominantly vegetable protein diet fed at 1.1 g/kg body weight is sufficient to cover the needs of most subjects in the group studied.