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Results


TABLE 1 Descriptive statistics for outcome variables by sex and type of supplement

Variable

Males

Females

Atole (n = 118)

Fresco (n = 127)

Atole (n = 116)

Fresco (n = 99)

Length at 3 y, cm

86.9 ± 3.8

85.3 ± 4.0*

86.3 ± 3.5

83.6 ± 3.6*

Weight at 3 y, kg

12.5 ± 1.2

11.8 ± 1.3*

12.1 ± 1.3

10.9 ± 1.1*

Height at adolescence, cm

157.5 ± 9.2

158.3 ± 7.6

150.5 ± 5.3

148.8 ± 4.7*

Weight at adolescence, kg

48.3 ± 8.1

49.6 ± 7.9

48.3 ± 6.7

46.0 ± 5.7*

Fat-free mass at adolescence, kg

41.9 ± 6.7

43.1 ± 6.4

37.3 ± 5.3

35.2 ± 4.6*

Values are means SD.
* P<0.05, t-test comparing Atole and Fresco. Length at 3 y of age did not differ between Atole and Fresco in 1968, at baseline (Habicht et al.1995, Ruel et al. 1992). Also note that maternal height (Table 2) did not differ by village type.

TABLE 2 Descriptive for independent variables by sex and village type

 

Males

Females


Atole

Fresco

Atole

Fresco

Variable



n


n


n


Supplement energy, KJ/day

118

536 ± 377

127

88 ± 75*

116

481 ± 322

99

71 ± 54*

Home diet energy, KJ/day²

118

2996 ± 912

126

3209 ± 971

113

2795 ± 787

97

2908 ± 1013

Supplement energy, kcal/day

118

128 ± 90

127

21 ± 18*

116

115 ± 77

99

17 ± 13*

Home diet energy, kcal/day²

115

716 + 218

126

767 ± 232

113

668 ± 188

97

695 ± 242

Maternal height, cm

116

148.8 ± 5.1

123

148.8 ± 5.1

114

149.0 ± 5.2

97

149.3 ± 5.6

SES³

116

-0.1 ± 0.9

122

-0.1 ± 0.8

114

-0.1 ± 0.9

97

0.0 ± 1.0

Time w/diarrhea, %

117

8.8 ± 8.2

126

7.7 ± 6.7

115

6.4 ± 6.2

97

7.9 ± 7.8

Maturation, years

116

16.1 ± 2.0

123

16.8 ± 1.9*

107

16.7 ± 1.8

97

16.7 ± 2.0

Age, years

118

16.1 ± 1.4

127

16.6 ± 1.5*

116

16.5 ± 1.4

99

16.4 ± 1.6

Duration of breastfeeding, months

113

19.1 ±6.9

121

19.3 ± 4.5

110

18.0 ± 5.6

94

19.7 ± 5.2*

Values are means ± SD.

* P<0.05, t-test comparing Atole and Fresco.

¹ Differences in sample sizes among variables are due to missing values.

² The percent with dietary energy intakes equal to the median or greater was 45.2%; (n = 115) and 54% (n = 126) in males and females respectively in Atole villages. The corresponding values for Fresco were 50.4% (n = 113) and 49.5% (n = 97). Differences between Atole and Fresco were not significant for males or females.

³ Standarized scores from factor analysis are unitless.

Descriptive statistics for outcome variables (Table 1) and independent variables (Table 2) are presented by sex and supplement type. Length and weight at 3 y of age were significantly greater in Atole compared with Fresco villages for both genders (P<0.05). Differences in favor of Atole villages were 1.6 and 2.7 cm in length and 0.7 and 1.2 kg in weight for males and females, respectively. Weight, height and FFM at adolescence were also significantly greater in Atole villages in females (P<0.05) but comparisons were not significantly different between Atole and Fresco villages in males. Chronological age as well as maturation were significantly greater in Fresco males (e.g., 16.1 and 16.6 y for Atole and Fresco males, respectively, for chronological age; Table 2); later analyses of size at adolescence adjust for these differences. Table 2 also shows that energy intake from the supplement was significantly greater in Atole villages (P<0.05) for both genders, and that energy intake from home diets, although slightly greater in Fresco villages, was not significantly greater. Maternal height, socioeconomic status and percent time with diarrhea did not differ between supplement types for either gender.

TABLE 3 Adjusted length/heicht (cm) of Atole and Fresco subjects

Gender, variable

Atole¹

Fresco²

Differences²

P

Males

Length at 3 y, cm

87.3 ± 0.3

85.3 ± 0.3

2.0

0.000

Height at adolescence, cm

158.6 ± 0.5

157.4 ± 0.5

1.2

0.111

Height at adolescence adjusted for length at 3 y, cm

157.5 ± 0.4

158.4 ± 0.4

-0.9

0.111

Females

Length at 3 y, cm

86.5 ± 0.3

83.6 ± 0.3

2.9

0.000

Height at adolescence, cm

150.7 ± 0.4

148.6 ± 0.5

2.1

0.001

Height at adolescence adjusted for length at 3 y, cm

149.6 ± 0.4

149.8 ± 0.4

-0.2

0.631

¹ Means ± SE. Means were adjusted for dietary intake, percent of time with diarrhea, socioeconomic status, maternal height and maturation (adolescence only).

² Atole minus Fresco.

Tables 3-5 present adjusted mean values of the various outcome variables for Atole and Fresco villages, by gender. The regression models (numbered consecutively from 1 to 18) used to compute these adjusted means are presented in Appendices 1-3.

TABLE 4 Adjusted weight (kg) Atole and Fresco subjects

Gender, variable

Atole¹

Fresco¹

Differences¹

P

Males

Weight at 3 y, kg

12.5 ± 0.1

11.8 ± 0.1

0.7

0.000

Weight at adolescence, kg

49.4 ± 0.5

48.2 ± 0.5

1.2

0.084

Weight at adolescence adjusted for weight at 3 y, kg

48.5 ± 0.4

49.1 ± 0.4

-0.6

0.273

Weight at adolescence adjusted for height at adolescence, kg

49.1 ± 0.4

48.6 ± 0.4

0.5

0.347

Females

Weight at 3 y, kg

12.2 ± 0.1

10.9 ± 0.1

1.3

0.000

Weight at adolescence, kg

48.2 ± 0.6

46.0 ± 0.6

2.2

0.009

Weight at adolescence adjusted for weight at 3 y, kg

46.7 ± 0.5

47.7 ± 0.5

-1.0

0.174

Weight at adolescence adjusted for height at adolescence, kg

47.8 ± 0.5

46.5 ± 0.6

1.3

0.109

¹ Means ± SE. Means were adjusted for dietary intake, percent time with diarrhea, socioeconomic status, maternal height and maturation (in the case of adolescence only).

² Atole minus Fresco

At 3 y of age, adjusted lengths and weights for both males and females were significantly greater in Atole compared with Fresco villages (Tables 3 and 4). In length, differences in adjusted means between Atole and Fresco were 2.0 cm in males and 2.9 cm in females, while in weight they were 0.7 kg and 1.3 kg for males and females, respectively. These differences were slightly greater in length compared with those obtained using unadjusted means (unadjusted differences were 1.6 and 2.7 cm, respectively in males and females; from data in Table 1) but nearly similar in the case of weight (unadjusted differences were 0.7 and 1.2 kg, respectively, in males and females; Table 11.

TABLE 5 Adjusted fat-free mass (FFM) in Atole and Fresco subjects

Gender, variable

Atole¹

Fresco¹

Differences²

P

Males

FFM, not adjusted for height, kg

42.8 ± 0.4

42.0 ± 0.4

0.8

0.162

FFM, adjusted for height, kg

42.5 ± 0.3

42.3 ± 0.3

0.2

0.641

Females

FFM, not adjusted for height, kg

37.3 ± 0.4

35.2 ± 0.5

2.1

0.002

FFM, adjusted for height, kg

36.9 ± 0.4

35.7 ± 0.4

1.2

0.052

¹ Means ± SE. Means were adjusted for dietary intake, percent time with diarrhea, socioeconomic status, maternal height and for maturation (in the case of adolescence only).

² Atole minus Fresco

Adolescents from Atole villages were taller (Table 3) and heavier (Table 4) than those of Fresco villages, although the differences were not statistically significant in males. The adjustments, particularly the correction for differences in maturation at adolescence between Atole and Fresco samples, reversed the sign of the anthropometric differences in males to favor Atole (i.e., Fresco males were taller and heavier at adolescence before adjustment but shorter and lighter after adjustment). Relative to adjusted differences at 3 y of age, adjusted differences at adolescence were smaller for height (1.2 versus 2.0 cm in males and 2.1 versus 2.9 cm in females; Table 3) but larger for weight (1.2 versus 0.7 kg in males and 2.2 versus 1.3 kg in females; Table 4)

Differences between Atole and Fresco villages at adolescence ceased to be significant after adjustment for length or weight at 3 y, indicating that differences observed at adolescence were due to differences already established at 3 y of age (Tables 3 and 4).

When weight at adolescence was adjusted for height at adolescence (Table 41).values - but differences in weight between Atole and Fresco villages were about half the size of the unadjusted values - but still substantial in females - and not statistically significant for either sex.

FFM at adolescence was greater in Atole villages in both males (0.8 kg) and females (2.1 kg). When FFM was adjusted for height, the Atole versus Fresco difference in males was reduced to 0.2 kg and became nonsignificant whereas that fin females was reduced to 1.2 kg but remained significant (Table 5).


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