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TABLE 1 (continued)

Identity No.

Subject (kg)

Weight (cm)

Height (%)

W/H index kcal/day

Energy Intake kcal/kg/day

(delta) Weight (kg)

44 54.1 168.5 82.7 3,200 59.1 0.2
45 55.8 164.6 86.0 3,200 57.3 - 0.3
46 62.7 164.0 98.7 3,200 51.0 0.1
48 57.8 166.7 89.5 3,200 55.4 0.6
49 64.0 161.7 103.7 3,200 50.0 0.1
51 61.0 161.0 99.8 3,200 52.5 - 0.8
52 68.4 173.8 98.6 3,200 46.8 - 0.2
53 64.5 174.5 92.5 3,200 49.6 - 0.2
54 67.0 169.5 101.2 3,200 47.8 - 0.2
55 64.8 170.5 96.9 3,200 49.4 - 0.5
56 56.7 164.0 90.1 3,200 56.4 - 0.1
57 51.8 161.8 83.9 3,200 61.8 0.8
58 55.8 160.0 92.2 3,200 57.3 0
59 73.2 180.8 97.6 3,200 47.8 - 1.5
60 63.7 168.6 96.7 3,200 50.2 0.5
Mean 62.4 166.1 96.7 3,231 52.4 - 0.13
SD 6.7 6.5 7.1 89 5.0 0.73

Anthropometric measurements such as body weight, height, waist, gluteal and mid-upper arm circumference, triceps fat-fold and subscapular fat-fold, thorax circumference and cranial circumference were obtained on days 1 and 11.

Summary of Main Results

Energy intake

The subjects received a mean daily energy intake of 3,247 104 kcal/day, or 52 5 kcal/kg/day.

Weight change Negligible, non-significant mean body weight changes were observed. About half of the individuals gained weight and the rest lost it, with an overall mean equal to - 0.128 0.729.

Urinary nitrogen intake: The mean urinary nitrogen excretion computed for the last five days was 143.5 14 mg/kg/day.

TABLE 2 Mean Daily Nitrogen Balances Resulting from a Chilean Mixed Diet Consumed for 10 Daysa

Subject Identity No

N Intake

Urinary N

Faecal N

N Balance

mg N/kg/day

1 183 139.3 35.7 + 3.0
2 183 165.9 19.4 - 73
3 183 156.7 31.5 - 10.2
4 183 136.2 36.9 + 4 9
5 182 144.0 25.0 + 8.0
6 183 136.6 33.2 + 8.2
7 183 156.2 28.1 - 6.3
8 183 135.0 37.6 + 54
9 182 123.6 44.1 + 9 3
10 182 118.7 22.7 + 35.6
11 183 126.4 34.2 + 174
12 183 141.2 44.3 - 75
13 183 158.8 16.7 + 2.5
14 182 141.0 29.4 + 6.6
15 183 142.6 28.0 + 74
16 183 152.0 36.7 - 10.7
17 183 156.1 37.6 - 15.6
18 182 159.3 30.5 - 12 8
19 185 159.6 34.5 - 14.1
20 183 164.9 27.9 - 1.6
21 183 137.4 35.4 + 5.2
22 182 142.5 35.1 - 0.6
24 187 149.6 23.7 + 8.7
25 183 125.4 35.0 + 17.6
26 182 135.6 30.4 + 110
27 182 158.7 31.9 - 13.6
28 182 129.0 34.8 + 13.2
29 182 124.2 27.3 + 25.5
30 182 147.8 28.4 + 0.8
31 182 146.0 30.3 + 07
33 183 133.8 21.6 + 22.6
35 182 159.5 20.3 - 2.8
36 183 133.6 23.5 + 20.9
37 183 155.1 24.3 - 1.4
38 182 153.7 23.1 + 0.2
39 182 158.4 29 2 + 10.6
42 183 141.4 20.6 + 16.0

TABLE 2. (continued)

Subject Identity No

N Intake

Urinary N

Faecal N

N Balance

mg N/kg/day

43 183 149.6 30.3 - 1.9
44 183 153.3 22.6 + 2.1
45 183 142.7 20.3 + 15.0
46 183 155.8 19.2 + 3.0
48 183 154.7 26.7 + 3.4
49 183 114.2 36.0 + 27.8
51 183 129.2 25.5 + 23.3
52 183 147.8 24.5 + 5.7
53 183 127.0 46.5 + 4.5
54 183 168.4 50.3 - 10.7
55 183 144.4 25.1 + 8.5
56 182 119.4 41.8 + 15.8
57 183 113.4 37.2 + 27.4
58 183 132.5 30.1 + 15.4
59 183 160.0 16.2 + 1.8
60 183 146.1 21.9 + 10.0
Mean 183 143.5 29.3 + 5.8
SD   14.1 7.3 11.7

Faecal nitrogen The faecal N excretion was 29.3 7.3 mg/kg/day. It is important to point out that this value is of the same magnitude found in our previous experiments in which male subjects consumed the same mixed diet in short-term and long-term experiments.

Nitrogen balance: The mean nitrogen balance for the population studied was + 5.8 11.7 mg N/kg/day (table 4).

Biochemical values in blood: All the parameters studied at the onset of the study fell within the normal range of values for young adult male subjects.

Anthropometric measurements: The anthropometric measurements made on days 1 and 11 failed to reveal significant changes in the parameters studied.

TABLE 3. Biochemical Measurements

Subject Identity No

Total Protein (g/dl)

Albumin (g/dl)

Globulin (g/dl)

A/G Index

Haematocrit (%)

Haemoglobin (g/dl)

1 7.7 4.8 2.9 1.6 49.3 16.9
2 6.3 4.0 2.3 1.7 43.0 14.3
3 7.3 5.0 5.5 1.9 44.5 15.3
4 6.3 4.2 2.1 2.0 45.2 15.3
5 7.0 5.2 1.8 2.8 46.9 16.1
6 6.3 4.0 2.2 1.8 42.8 14.5
7 6.9 5.1 1.8 2.8 46.7 16.1
8 6.1 4.4 1.7 2.5 46.8 16.5
9 6.7 4.4 2.3 1.9 44.8 15.5
10 7.3 4.9 2.4 2.1 44.1 15.4
11 7.1 4.5 2.6 1.8 45.0 15.6
12 7.1 4.9 2.2 2.3 39.1 13.6
13 6.5 4.1 2.3 1.8 46.5 16.0
14 6.3 3.9 2.4 1.7 43.6 15.0
15 6.7 4.5 2.2 2.0 48.1 16.4
16 7.0 4.7 2.3 2.1 45.4 16.1
17 6.7 4.7 2.0 2.3 45.7 15.7
18 7.7 5.2 2.5 2.1 44.4 15.4
19 6.8 4.4 2.8 1.8 45.8 15.9
20 6.7 3.4 2.2 1.1 46.7 16.1
21 6.9 4.2 2.7 1.5 43.3 14.4
22 6.3 4.4 1.9 2.3 46.8 15.3
24 6.5 4.1 2.4 1.7 49.6 16.6
25 7.0 4.2 2.8 1.5 43.1 14.9
26 7.0 4.7 2.4 2.0 47.7 16.2
27 7.1 5.0 2.1 2.5 47.0 16.4
28 6.0 4.4 1.6 2.7 49.1 16.8
29 6.5 4.7 1.7 2.8 46.5 16.0
30 6.5 4.1 2.4 1.7 44.5 15.2
33 7.6 4.7 2.9 1.6 45.4 15.3
35 7.8 4.6 3.2 1.4 45.6 14.9
36 7.0 4.6 2.4 1.9 42.2 13.5
37 7.0 4.4 2.5 1.8 45.0 15.5
38 7.0 4.6 2.4 1.9 43.8 14.7
39 7.6 4.6 3.0 1.5 43.4 15.0
42 6.2 3.9 2.3 1.7 41.8 14.1
43 7.0 4.9 2.1 2.3 46.2 16.3
44 6.2 4.4 1.8 2.4 39.6 13.3
45 6.4 3.9 2.4 1.6 42.2 14.1

TABLE 3. (continued)

Subject identity No

Total a Protein (g/dl)

Albumin (g/dl)

Globulin (g/dl)

A/G Index

Haematocrit (%)

Haemoglobin (g/dl)

46 6.7 4.6 2.0 2.3 38.1 1 3.0
48 7.0 4.1 2.9 1.4 47.8 1 6.5
49 6.8 3.4 3.4 1.0
51 7.0 4. 1 2.9 1 .4 47.2 15.8
52 7.3 4.1 3.2 1.3 43.6 14.9
53 6.8 3.7 3.1 1.2 44.4 14.7
54 7.2 3.3 3.9 0.8
55 6.2 4.4 1 .8 2.4 39.8 1 3.4
56 6.8 3.3 3.5 0.9 43.4 14.4
57 7.4 4.0 3.4 1 .2 43.8 1 5.7
58 6.8 3.5 3.2 1.1 46.2 16.5
59 7.4 3.9 3.5 1.1
60 7.6 4. 1 3.5 1 .2 41 .6 1 4.7
Mean 6.8 4.3 2.6 2.0 44.7 1 5.3
SD 0.5 0.5 0.7 1.5 2.6 1 0

TABLE 4. Distribution of Nitrogen Balance

N Balance (mg/kg) No. % Cumulative
      No. %
>= + 10.0 17 32.1 17 32.1
+ 5.0 to 9.9 10 18.9 27 50.9
0.0 to 4.9 11 20.7 38 71.7
- 5.0 to - 0.1 5 9.4 43 81.1
- 10.0 to - 5.1 3 5.7 46 86.8
< - 10.0 7 13.2 53 100.0

Conclusions and Comments

Mean daily N balance was slightly positive for the group, but seven of 53 individuals mantained on 1.1 9 of protein per kg were in significant negative N balance (lower than - 10 mg N/kg/day).

Subjects with the largest positive N balance corresponded to those with highest energy intakes.

The faecal N excretion level was comparable to that in our previous data using similar diets and subjects.

Subjects appeared to be in energy balance based on weight and anthropometric measurements.

A predominantly vegetable protein diet fed at 1.1 g/kg body weight is sufficient to cover the needs of most subjects in the group studied.


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